498 The effects of commercial transwell-system and six different types of the medium on the expansion of the 3D-printed collagen scaffolds loaded with cryopreserved patient-derived melanoma explants

A previous study from our laboratory demonstrated the effects of in vitro three-dimensional(3D)-Printed collagen scaffolds on maintenance cryopreserved patient-derived melanoma explants. However, it remains unknown precisely whether culture condition influences 3D-printed loaded with explant(3D-PCS-PDME). To optimize conditions 3D-PCS-PDME), were manufactured a 3DX bioprinter. The scaffold-on-frame construction was Fragments PDMEs (approximately 1- 2 mm) and incubated with/without transwell chamber six different types media (DMEM high-glucose; MEM a; DMEM/F12; RPMI1640; fibroblast basal medium (FBM); mesenchymal stem cell growth medium) for 7 days. results reveal that a, DMEM/F12, FBM provides good environment maintaining 3D-PCS-PDME, compared typical method. In addition, stability 3D-PCS-PDME under method shows higher than Taken together, findings suggest optimization might serve as an important factor building up 3D alternative animal-free testing its microenvironment using